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DTSTAMP:20261021T153000Z
STATUS:CONFIRMED
DTSTART:20261021T153000Z
DTEND:20261021T170000Z
SUMMARY: Seminaire: Laure Teysset and Andana Barrios
DESCRIPTION:TBC 

 Abstract:
 TBC and The lncRNA BAMBOO decoys NF-YA2 transcription factor from the promoter of its root-specific targets in Arabidopsis Abstract:
 Plant developmental plasticity emerges from transcriptional reprogramming in response to environmental shifts, which largely depends on the activity of transcription factors (TFs). In Arabidopsis thaliana, CCAAT-binding TFs NF-YA2 and NF-YA10 (Nuclear Factor-Y A2 and A10) are post-transcriptionally regulated by microRNAs, participating in a regulatory hub controlling root architecture. Here, RNA-seq and ChIP-seq approaches served to identify NF-YA2 direct target genes involved in lateral root (LR) development, water deprivation and hormone pathways. Furthermore, we searched for NF-YA2 potential RNA partners by using RIP-seq and identified a long noncoding RNA (lncRNA) named BAMBOO after its counterpart PANDA, which interacts with mammalian NF-YA. BAMBOO is dynamically regulated during LR development and bamboomutant plants exhibit a NF-YA2-related root developmental phenotype. bamboo plants share half of its deregulated genes with the NF-YA2 regulome. In vivo, in vitro and in silico assays indicated that NF-YA2 - BAMBOO interaction depends on the DNA-binding domain and RNA sequence. When overexpressed, the lncRNA BAMBOO disturbed NF-YA2 binding to its target promoters suggesting an RNA-based competition of target recognition by NF-YA2 in chromatin. Hence, the study of lncRNAs at the organism level underscores their capacity to intricately modulate TF activity throughout targeted developmental processes, thereby fine-tuning regulatory networks.
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 Abstract:
 TBC and The lncRNA BAMBOO decoys NF-YA2 transcription factor from the promoter of its root-specific targets in Arabidopsis Abstract:
 Plant developmental plasticity emerges from transcriptional reprogramming in response to environmental shifts, which largely depends on the activity of transcription factors (TFs). In Arabidopsis thaliana, CCAAT-binding TFs NF-YA2 and NF-YA10 (Nuclear Factor-Y A2 and A10) are post-transcriptionally regulated by microRNAs, participating in a regulatory hub controlling root architecture. Here, RNA-seq and ChIP-seq approaches served to identify NF-YA2 direct target genes involved in lateral root (LR) development, water deprivation and hormone pathways. Furthermore, we searched for NF-YA2 potential RNA partners by using RIP-seq and identified a long noncoding RNA (lncRNA) named BAMBOO after its counterpart PANDA, which interacts with mammalian NF-YA. BAMBOO is dynamically regulated during LR development and bamboomutant plants exhibit a NF-YA2-related root developmental phenotype. bamboo plants share half of its deregulated genes with the NF-YA2 regulome. In vivo, in vitro and in silico assays indicated that NF-YA2 - BAMBOO interaction depends on the DNA-binding domain and RNA sequence. When overexpressed, the lncRNA BAMBOO disturbed NF-YA2 binding to its target promoters suggesting an RNA-based competition of target recognition by NF-YA2 in chromatin. Hence, the study of lncRNAs at the organism level underscores their capacity to intricately modulate TF activity throughout targeted developmental processes, thereby fine-tuning regulatory networks.
LOCATION:Autres Instituts - Auditorium Gallois - 45 rue d Ulm, 75005 Paris
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