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Mercredi 15 Janvier 2025    12:00
Invite par: Pierre-Marie Lledo and Philippe Sansonetti Cytomegalovirus (CMV) infection of the foetus is a major cause of intellectual deficiency and other neurodevelopmental deficits as well as of hearing loss and visual impairment. We will review human postmortem data as well as experimental studies aiming at understanding the pathophysiology of CMV-induced encephalopathy and internal ear defects.
The speaker is invited by Pierre-Marie Lledo and Philippe Sansonetti as part of joint seminars organized by the Department of Neuroscience and the Cross-disciplinary Research Program in Maternal and Child Health. Plus d'infos...
Tags: CMV, Encephalopathy, Infections specific to the perinatal period, HIV/AIDS, Congenital cytomegalovirus infection
Tags: Hepatitis B virus, Viral structural proteins, Hepatitis
Annonce publiée le 11-01-2025
Centre de Recherche sur l'Inflammation
salle 241B, Faculte de Medecine site Bichat, 16 rue Henri Huchard - 75018
Jeudi 16 Janvier 2025    9:00
The two GDRs AQV (Quantitative approaches to living systems) and IMABIO (Imaging in Biology) are organising a joint workshop on 16 January 2025 at the Institut Jacques Monod in Paris on new microscopies in cell biology. The aim is to bring together scientists from biology, physics, engineering and data science to discuss new imaging strategies for : measuring physical descriptors of cellular organisation and function; improving imaging resolution; making smart microscopes more effective at capturing processes of interest. Confirmed speakers : Juliette Azimzadeh, Institut Jacques Monod, Paris Victor Barolle, Institut Langevin, Paris Ricardo Henriques, Instituto Gulbenkian de Ciencia, Oeiras and University College London Kheya Sengupta, Interdisciplinary Centre for Nanoscience, Marseille Vincent Studer, Interdisciplinary Neuroscience Institute, Bordeaux Ilaria Testa, KTH Royal Institute of Technology, Stockholm Oral presentations and posters will also be selected from the abstracts submitted. Registration is free but compulsory and is now open here: https://newmic4cellbio2.sciencesconf.org/ The organisers
Pierre Bon, Nicolas Borghi, Cecile Leduc, Loic Le Goff, Pierre-Henri Puech Plus d'infos...
Tags: Institut Jacques Monod, Jacques Monod
Annonce publiée le 11-11-2024
Institut Jacques Monod
Institut Jacques Monod Amphitheatre Buffon, 15 rue Helene Brion, Paris, France
Centre de recherche - Paris - Amphitheatre Helene Martel-Massignac (BDD)
Jeudi 16 Janvier 2025    12:00
Invite par: Jessica Bryant Abstract : Unlike model organisms, malaria parasites divide in unconventional ways producing not two but dozens of daughter cells, in a single cell cycle round. This points to a yet-to-be-explored original and divergent cell ... Plus d'infos...
Invitee par l’equipe Konstantinides, Zayna Chaker (Institute of Functional Genomics of Lyon (IGFL) Ens de Lyon, CNRS) va presenter un Seminaire de l’Institut Jacques Monod sur le theme : Spatio-Temporal coordination of neural stem cells in the adult brain: A functional and evolutionary perspective Resume : Research over the last three decades has demonstrated that new neurons can be generated in the adult brain, and integrate into pre-existing complex circuits. The process of adult neurogenesis is evolutionary conserved among vertebrates, including fishes, frogs, reptiles, birds, rodents and primates. It is sustained by a small population of undifferentiated cells, called neural stem cells (NSCs), which persist as embryonic vestiges in adult brains. These cells reside in tightly controlled micro-environments called niches. The addition of young cells constitutes an important layer of adult brain plasticity, further enhancing its ability to adapt to diverse life experiences.However, the physiological relevance of adult-born neurons as well as the regenerative power of NSCs after injury are still highly debated to date, especially in mammals. Besides constitutive neurogenesis, we recently showed that regionally-defined subpopulations of NSCs in the adult ventricular niche produce transient waves of functionally-relevant interneurons in response to pregnancy and motherhood. This process finely tunes the mother's olfactory sensitivity to own versus alien pup odor (Chaker et al. 2023). In my lab, we are now investigating the cellular and molecular mechanisms allowing regionally-distinct pools of NSCs to coordinate their behavior in space (across all niches) and time (from embryo to different phases in adulthood), under specific physiological and pathological conditions. Plus d'infos...
Annonce publiée le 04-01-2025
Institut Jacques Monod
Insitut Jacques Monod
Vendredi 17 Janvier 2025    12:00
Invite par: Fabiola Terzi Philippos Mourikis is a Research Director (DR1) at CNRS, leading a group within the team of Prof. F. Relaix since 2019. Recently appointed as a Team Leader at the Institut Necker-Enfants Malades (INEM), Dr. Mourikis will transfer his group to INEM in January 2025.With a long-standing interest in Notch signalling and the regulation of stem cell quiescence, Philippos Mourikis uses mouse skeletal muscle as a model system. His research integrates high-throughput technologies and mouse genetics to uncover novel regulators of muscle stem cell establishment and maintenance.Mourikis earned his PhD (20002005) at Harvard Medical School under the mentorship of Prof. Artavanis-Tsakonas, who famously cloned the Notch locus. During his doctoral studies, he explored ageing and Notch signalling in Drosophila melanogaster. His introduction into mouse genetics and muscle research began during his postdoctoral fellowship in the lab of Prof. Tajbakhsh at the Pasteur Institute, Paris (2006-2012). This was followed by a second postdoctoral position in Prof. Relaix's team at the Institute of Myology, also in Paris, where PM secured a CR1 CNRS position in 2014.PM EMBO Muscle Meeting, Chateau de Montvillargene, France (2022)
1st Symposium on "Muscle Stem Cells in Growth and Disease," Osaka, Japan (2023)
Frontiers in Myogenesis Conference, Sao Paulo, Brazil (2023)
The Notch Meeting XI, Athens, Greece (2019)
The Notch Meeting XII, Athens, Greece (2023)
The Notch Meeting XIII, Athens, Greece (2025, upcoming)
Seminar topic: Notch-driven autocrine signalling networks maintain quiescence in muscle stem cells Plus d'infos...
Invite par: 2024-2025 EEB external seminar We study the time to the most recent common ancestor of a sample of finite size in a wide class of genealogical models for populations with variable size. This is made possible by recently developed results on inhomogeneous phase-type random variables, allowing us to obtain the density function and the moments(expectation and variance) of the TMRCA of time-dependent coalescent processes in terms of matrix formulas.
A careful study of this mathematical theory with applications to classical statistics of population genetics, such as the TMRCA or the site frequency spectrum, will be provided. This may be useful to determine if the population under study suffered phenomena such as exponential growth, recurrent bottlenecks, skewed offspring or selection.... Plus d'infos...
Tags: Evolutionary biology, Population genetics, Phylogenetics, Genetic genealogy, Human evolution, Most recent common ancestor, Population bottleneck, Viral phylodynamics
Annonce publiée le 04-01-2025
IBENS
salle Favard, IBENS 46 rue d'Ulm 75005 Paris
Vendredi 17 Janvier 2025    14:00
Invite par: Laure Bally-Cuif and Philippe Sansonetti Plus d'infos...
Laboratoire Jean Perrin - Campus Jussieu - T 22-32- 4e et. - P407
Mardi 21 Janvier 2025    11:45
Invite par l’Institut Jacques Monod, Lucas Alves Tavares (Center for Virology Research and Department of Cell and Molecular Biology, University of Sao Paulo (USP) & Institut Curie, Paris) va presenter un Paris Postdoc Seminar sur le theme : Unverealing the role of AP-1?2 in the Endosome Maturation and Extracellular Vesicles Release Resume : The adaptor protein (AP) complexes are heterotetrameric complexes that coordinate protein trafficking in the endocytic and secretory pathways. AP-1, a complex of four distinct subunits (?, ?1, ?1, and ?1), is thought to mediate protein trafficking between the trans-Golgi network (TGN) and endosomes and Lysosomes Related Organelles (LROs) though clathrin-coated vesicles (CCV). The human genome encodes two isoforms of the ?-adaptin (?1 and ?2) subunit that form two variants of AP-1 (AP-1?1 and AP-1?2). Previous studies demonstrated that ?2 may form an AP-1 complex variant, but the knowledge is limited regarding the cellular roles played by this alternative AP-1 complex. We previously reported that ?2 is part of a functional AP-1 complex variant hijacked by HIV-1 Nef for targeting CD4 and MHC-I for lysosomal degradation. Moreover, we recently demonstrated that the retrograde transport of CI-MPR and ATP7B requires AP-1?2. During my internship at Graca Raposo laboratory (Institut Curie), we are investigating the participation of AP-1?2 in the maturation of early endosomes to late endosomes and also for the Extracellular Vesicles (EVs) secretion by using immunofluorescence, nanoparticle tracking analysis, western blot, immuno-electron microscopy analysis and conventional transmission electron microscopy analysis. Therefore, this study has the potential to contribute to the understanding of fundamental processes in the regulation of protein and membrane trafficking involved in the endosome maturation and EVs shedding. Plus d'infos...
Annonce publiée le 04-01-2025
Institut Jacques Monod
Institut Jacques Monod Salle Francois Jacob, 15 rue Helene Brion, Paris, France
Jeremy Salle (equipe Minc) va defendre son Habilitation a Diriger les Recherches : « Geometrie des clivages embryonnaires : Mecanismes et Implications » La soutenance se tiendra en francais le mardi 21 janvier 2025 a 14h00, en salle Francois Jacob (IJM), en presence du jury compose de : Jenifer Croce (LBDV – Villefranche-sur-Mer) Remi Dumollard (LBDV – Villefranche-sur-Mer) Patrick Lemaire (CRBM – Montpellier) Lionel Pintard (IJM – Paris) Marie-Helene Verlhac (College de France – Paris) Nicolas Minc (IJM – Paris) Plus d'infos...
Annonce publiée le 04-01-2025
Institut Jacques Monod
Institut Jacques Monod Salle Francois Jacob, 15 rue Helene Brion, Paris, France
salle 241B, Faculte de Medecine site Bichat, 16 rue Henri Huchard - 75018
Jeudi 23 Janvier 2025    12:30
Summary: Type 1 diabetes (T1D) is classically viewed as an autoimmune disease. While this certainly still holds true, recent work by us and others have highlighted a universal state of “benign” autoimmunity present in all individuals. The key question is therefore to understand the mechanisms by which this benign autoimmunity progresses towards an aggressive state and leads to T1D. Two non-mutually exclusive mechanisms are at play: defective immune regulation and an increased vulnerability of beta cells to the autoimmune attack. These mechanisms also provide relevant therapeutic targets that we are exploring in preclinical mouse and in-vitro human models and in patients. Overall, T1D should now be viewed as both an autoimmune and a beta-cell disease, and should be treated accordingly. Biography: R. Mallone received his MD PhD degree from the University of Turin, Italy. After a Postdoc with Jerry Nepom at the Benaroya Institute in Seattle, he moved to Paris where he is currently Professor of Immunology at Université Paris Cité, Diabetologist at the Cochin Hospital and leads a research team at the INSERM Cochin Institute. He is also director of a satellite research lab at the Indiana Biosciences Research Institute in Indianapolis. His research spans from preclinical studies with human samples and mouse models to clinical trials. It focuses on autoimmune T cells and their dialogue with pancreatic beta cells to understanding type 1 diabetes mechanisms and develop novel biomarkers and therapeutics. Plus d'infos...
Tags: Immunology, Autoimmune diseases, T cells, Type 1 diabetes, Autoimmunity, Autoimmune disease, Beta cell, Biobreeding rat, Breakthrough T1D
Annonce publiée le 22-12-2024
Centre de Recherche des Cordeliers
Amphi Gustave Roussy
Vendredi 24 Janvier 2025    11:00
Bart N. Lambrecht, VIB Center for Inflammation Research, Ghent, Belgium IRF4 and IRF8 are key transcription factors controlling monocyte and DC development and function. In this talk, I will give 2 examples on how these TFs can control key aspects of macrophage homeostasis and disease. Mice deficient in IRF8 fail to develop cDC1 and monocytes and have a myeloproliferative syndrome. By disentangling the effects of IRF8 deficiency, we report that the well-known accumulation of neutrophils in Irf8-/- mice requires two independent hits; IRF8 must be simultaneously absent from myeloid progenitors as well as from mature macrophages. Whereas loss of IRF8 promotes the generation of neutrophils from progenitors, macrophage-specific loss of IRF8 and its downstream target FLT3 impaired their trans surveillance ability to consume FLT3L, the growth factor driving neutrophil accumulation. As neutrophils and macrophages represent alternative fates downstream of granulocyte-macrophage progenitors, we propose that cell fate decisions are accompanied by the institution of counting? mechanisms, in the form of growth factor regulation by cells of opposing fates to maintain stable cell ratios across the organism. Tissue resident alveolar macrophages (trAMs) are generally considered as resilient immunoregulatory cells that safeguard the alveolar space from overzealous inflammation that would compromise gas exchange. Using mice in which trAMs can be specifically deleted and replaced, we show that trAMs can also act as pro-inflammatory cells that orchestrate type-2 immunity in the lungs. After house dust mite allergen inhalation, the alarmin IL-33 primes ILC2s to produce IL-13 which reprograms trAMs by upregulation of IRF4. While trAM identity is rapidly lost due to chromatin remodeling and downregulation of PPARg dependent homeostatic genes, IRF4-dependent binding and transcription at loci coding for chemokines and cell-cell fusion machinery leads to recruitment of granulocytes, ILC2s and Tregs to the alveolar space, and to formation of multinucleated giant cells. Therefore, trAMs are highly versatile cells that tightly control recruitment of inflammatory cells to the alveolar space and can be reprogrammed to drive lung inflammation via IRF4. Plus d'infos...
Hopital site de Paris - Amphitheatre Constant-Burg - 12 rue Lhomond, Paris 5e
Vendredi 24 Janvier 2025    12:00
Invite par: Vincent Goffin - GH and PRL pathophysiology Wilbert Zwart completed his PhD at the Netherlands Cancer institute (NKI; Amsterdam) in 2009, and received is post-doctoral training at the CRUK-CI in Cambridge (UK), in the lab of prof. Jason Carroll. Since 2011, Wilbert runs his independent research lab at the NKI studying hormone-dependent cancers, focusing on hormone receptor biology, epigenetics and gene regulation. His projects have a full circleFunctional Genomics in Oncology Hormones, Epigenetics and Cancer: From Clinical Trials to Molecular Mechanisms and Back Again
Plus d'infos...
Tags: Cancer, Oncology
Annonce publiée le 29-11-2024
Institut Necker Enfants Malades
Auditorium 3
Vendredi 24 Janvier 2025    12:00
Invite par: Helene MORLON - Section Ecologie et Biologie de l’Evolution Abstract : Plants have evolved a wide variety of life history strategies, from annual plants that complete their life cycle in a year to long-lived trees that can take decades to reach reproductive maturity. We demonstrate through stochastic modeling that this variation has significant consequences for community assembly and biodiversity. Applying this approach to a neotropical forest plot in Barro Colorado Island, Panama, we can explain fluctuations in species abundances across a large and diverse system using only census and life history data. Plus d'infos...
Tags: Population genetics, Evolution, Biodiversity, Genetic variation
Tags: Olfactory system, Olfactory receptors, Sensory receptors, Integral membrane proteins, Protein families, Richard Lounsbery Award
Annonce publiée le 18-12-2024
Institut Curie
Centre de recherche - Paris - Amphitheatre Marie Curie
Jeudi 30 Janvier 2025    0:00
Three researchers recruited by CNRS or Inserm in 2024 will present their research projects: Clotilde Cadart, Caroline Eozenou, Suzanne Faure-Dupuy. Plus d'infos...
Tags: French National Centre for Scientific Research, Inserm, Dupuy
Annonce publiée le 30-10-2024
Institut Cochin
Salle Rosalind Franklin
Vendredi 31 Janvier 2025    0:00
Ana-Maria Lennon Duménil is invited by Fatah Ouaaz. Plus d'infos...
Invite par: Fabiola Terzi Philippe Pierre is an international leader in dendritic cell biology and innate immunity research. During his PhD at the EMBL (Heidelberg, Germany), his research focused on the characterization of the microtubule-binding protein CLIP-170. After a post-doctoral research period at Yale University School of Medicine (USA), he focused on MHC II-restricted antigen presentation and immune response. In 2000, he created his laboratory at the Centre d 2015 Prize" for molecular immunology from the French Academy of Sciences. He was the director of the CIML until 2024 and is professor-adjunct of the University of Aveiro (IBiMED, Portugal) and the Shanghai Institute of Immunology (SII, PRC).Seminar topic: Functional consequences of endo-lysosomes mispositioning in phagocytes: A RUFY3/4 story! Plus d'infos...
Tags: Immune system, Vesicles, Centre d'immunologie de Marseille-Luminy, Phagocyte, Lysosome, Immunology, Dendritic cell, Innate immune system
Invite par: Gilles Crambert Summary: Solid tumors are heterogeneous environments, containing niches of varying hypoxia, acidosis, and nutrient deprivation. Evidence from us and others shows that such hostile conditions can endow cancer cells with highly aggressive traits, including increased growth, invasiveness, and rewired metabolism. Such niches can also support cancer stem cells and protect cancer cells against chemotherapeutic and immune-oncological therapies. Accordingly, we recently demonstrated that acid-adapted pancreatic cancer cells give rise to highly aggressive tumors with increased metastatic potential in vivo. Understanding these niches and their impact can uncover new targets for improving treatment of aggressive cancers. I will present our recent work in which we combine in vitro and in vivo cancer models, microfluidics, and spatial transcriptomics to study the mechanisms through which tumor acidosis may drive cancer aggressiveness. Plus d'infos...
Tags: 5th arrondissement of Paris, National Museum of Natural History, France, Triune brain
Annonce publiée le 21-12-2024
NeuroPSI
Salle de conference Albe-Fessard
Vendredi 07 Fevrier 2025    11:45
Levendredi 7 fevrier 2025, Sophie G. Martin (Department of Molecular and Cellular Biology, University of Geneva, Switzerland) presentera une Conference de l'Institut Jacques Monod sur le theme : Signaling and actin focus architecture for cell-cell fusion Resume : Sexual reproduction is ubiquitous amongst eukaryotes. This requires alternation of cell-cell (gamete) fusion and genome reduction through meiosis. My lab has been using the yeast sexual reproduction pathway to study how cells polarize to find a mate and mount a fusion reaction. In the fission yeast Schizosaccharomyces pombe, sexual reproduction occurs between P and M cells, which communicate through pheromone-GPCR-MAPK signaling, driving the formation of cell pairs. Transition from gametes to zygote involves local cell wall digestion at the point of gamete contact, while preserving cell integrity. We have shown that cell-cell fusion requires the actin fusion focus, an aster-like assembly of linear actin filaments assembled by the formin Fus1, which concentrates both signaling molecules and secretory vesicles carrying cell wall digestion enyzmes. I will present our recent work on the molecular mechanisms of formation of the actin fusion focus, which require both formation of a formin biomolecular condensate and cytoskeletal focusing through formin-myosin feedback. I will also describe our progress in understanding the roles of local MAPK and PAK signaling for cells to pierce their cell wall once and only once. Plus d'infos...
Annonce publiée le 04-01-2025
Institut Jacques Monod
Institut Jacques Monod Amphitheatre Buffon, 15 rue Helene Brion, Paris, France
The swimming motion of bacteria has recently seen an increasing interest from physicists, utilizing the recent progresses in microscopy and the development of microfluidics to study this interdisciplinary subject. In this seminar, I will present our two main experimental findings on the motile soil bacteria Burkholderia contaminans : its reaction to oxygen and its ability to gather micron-sized beads in clusters. First, we quantitatively studied its aerotaxis, i.e. its behaviour in an oxygen gradient. We notably characterized the aerotactic coefficient dependency on the oxygen concentration, with both a macroscopic (population scale analysis) and microscopic approaches (bacterial scale analysis), and compared it to the literature. Second, we uncovered a rich clustering phenomena happening when some micron-sized passive beads ( 2 - 40 mu; m ) are added to the bacterial suspension. Besides the enhanced bead diffusivity, the swimming bacteria are also responsible for a short-range attractive force between the beads. This results in a dynamical clustering of the beads, with a dynamics similar to Ostwald ripening and a characteristic cluster size slowly growing in t 1 / 3 without any apparent saturation. Plus d'infos...
Laboratoire Jean Perrin - Campus Jussieu - T 22-32- 4e et. - P407
Mardi 11 Fevrier 2025    11:45
Le mardi 11 fevrier, Richard Benton (Center for Integrative Genomics, University of Lausanne) presentera une Conference de l’Institut Jacques Monod sur le theme : Fatal chemosensation, and how insects fight back Resume : Insecticide resistance is a widespread challenge for the management of vectors transmitting pathogens and agricultural pests, requiring a better understanding of the genetic mechanisms underlying the evolution of resistance. Drosophila sechellia is a compelling model for such studies as it naturally evolved resistance to octanoic acid, an abundant chemical of its noni fruit host that is toxic for other insects, including close relatives D. simulans and D. melanogaster. We have used a multi-pronged strategy to identify genes contributing to octanoic acid resistance. We began by experimentally-evolving D. simulans strains with higher tolerance to octanoic acid and determined the resulting genetic architecture. To identify specific candidate genes, we integrated this analysis with a genome-wide association study of octanoic acid resistance in D. simulans and a genome-wide CRISPR selection screen upon octanoic acid exposure in D. melanogaster S2R+ cultured cells. We identified four candidates, with diverse predicted molecular and expression properties, and validated their relevance using genetic analyses in D. melanogaster. Two of these genes displayed an increased expression in the experimentally-evolved strains, paralleling their higher levels of expression in D. sechellia. Our results suggest an adaptive role of these genes in shaping toxin resistance both under laboratory conditions and during D. sechellia's evolutionary history. Plus d'infos...
Annonce publiée le 04-01-2025
Institut Jacques Monod
Batiment Condorcet Amphitheatre Pierre Gilles de Gennes, 4 rue Elsa Morante, Paris, France
au College de France - Salle D2 acces restreint, merci de passer par l'accueil du CDF : 11 place Marcelin Berthelot - 75005 Paris
Jeudi 13 Fevrier 2025    0:00
Anti-PD-1 therapy targets intratumoral CD8+ T cells to promote clinical responses in cancer patients. Recent evidence has suggested that anti-PD-1 also act in the periphery. In particular, new T cell clonotypes emerge during anti-PD-1 therapy within the tumor microenvironment, suggesting de novo priming in the periphery. However, the underlying mechanism remains incompletely understood. In this presentation, I will show the importance of TDLN during anti-PD-1 therapy and discuss unexpected mechanisms for the peripheral activity of anti-PD-1 antibodies Paris Post-docs seminar series. Plus d'infos...
Tags: Immune system, Immunology, Clusters of differentiation, Programmed cell death protein 1, Tumor microenvironment, T cell, Pd1, Antibody, Cancer immunotherapy, Dario Angelo Alberto Vignali
Invite par l’equipe Courtier, Peter Andolfatto (Professor, Dept. of Biological Sciences, Columbia University) presentera un seminaire de l’Institut Jacques Monod sur le theme : The evolution of toxin-resistant Na+,K+-ATPases: new insights from frogs and fireflies We study the process of adaptive evolution through the lens of repeated adaptation of many distantly species to a similar selection pressure (i.e. «parallel evolution»). Over the past decade, we have explored patterns of adaptation in the context of animals that have specialized in eating plants, or other animals, that contain toxic cardiotonic steroids (CTS). CTS are toxic to animals because they inhibit sodium-potassium ATPase, a key enzyme in animals needed in everything from maintaining cell homeostasis, muscle contraction to neuron activity. Here I review our most recent work combining comparative molecular evolution, molecular and biochemical assays andin vivoengineering of Drosophila to deduce the rules governing the adaptive evolution of CTS resistance in animals. Together, our results have interesting implications for how epistasis and pleiotropy both limit the rate of adaptive protein evolution and increase its predictability. Plus d'infos...
Annonce publiée le 04-01-2025
Institut Jacques Monod
Institut Jacques Monod Salle Francois Jacob, 15 rue Helene Brion, Paris, France
Lundi 17 Fevrier 2025    13:00
Save the date! The Single Cell Initiative, the Cell and Tissue Imaging Platform (PICT) and the Experimental Pathology (PATHEX) core facilities co-organise an afternoon dedicated to spatial omics at Institut Curie. Meet the core facilities and discover how your colleagues apply spatial omics technologies in their research! Agenda, registration link and more information to come soon. Plus d'infos...
Tags: Genomics, Omics, OMICS Publishing Group
Annonce publiée le 06-12-2024
Institut Curie
Centre de recherche - Paris - Amphitheatre Helene Martel-Massignac (BDD)
Mitochondria are double membrane-bound organelles that perform biosynthetic and signaling roles to control the life and death of the cell. Mitochondria are paramount to the metabolism and survival of cardiomyocytes, which have the most densely packed inner mitochondrial membrane of all cells. Cardiomyocytes are exquisitely sensitive to perturbations of mitochondrial structure, which can lead trigger downstream maladaptive and compensatory responses. In my talk, I will share our latest, unpublished findings using mouse models we have developed that have revealed the importance of inner mitochondrial membrane integrity in restraining cardiac inflammation and the emerging effects that biological sex can have on these phenomena. Timothy Wai is invited by Molly Ingersoll and Catherine Postic. Plus d'infos...
Centre de recherche - Paris - Amphitheatre Constant-Burg - 12 rue Lhomond, Paris 5e
Mercredi 12 Mars 2025    11:30
Proteins in cells are not homogeneously distributed, but often localized to specific compartments, which may or may not be enclosed by membranes. Here I will first discuss the dynamic formation of membraneless compartments in the non-equilibrium environment of living cells and how this formation can be regulated, e.g. by kinases [1]. Then I will show how single-molecule FRET (smFRET) and tracking of biomolecules can be used to study conformational dynamics and time-resolved cellular localization in living cells [2]. Results with the transcription initiation factor TAF2 show that the same protein can be in different compartments and thereby have different functions [3]. For the heat shock protein Hsp90 we will determine how proteins may be recruited to different compartments and have different conformational dynamics. Altogether, smFRET has the potential to change our view on compartment-specific protein dynamics and therefore signalling within living cells.
[1] C. Lan, J. Kim, S. Ulferts, F. Aprile-Garcia, A. Anandamurugan, R. Grosse, R. Sawarkar, A. Reinhardt and T. Hugel, Nat. Commun., 14:4831 2023 (https://doi.org/10.1038/s41467-023-40540-2) [2] A. Anandamurugan, A. Eidloth, P. Wortmann, L. Schrangl, F. Aprile-Garcia, C. Lan, R. Sawarkar, G. J. Schütz, T. Hugel, bioRxiv 2023 (https://doi.org/10.1101/2023.09.15.557875) [3] T. Bhuiyan, P. K. Mendoza Sanchez, N. Arecco, J. Kim, S. Nizamuddin, A. Prunotto, M. Tekman, M. L. Biniossek, S. Koidl, T. Hugel, S. J. Arnold, bioRxiv 2024, (https://doi.org/10.1101/2024.02.05.578926)
Tags: J. William Harbour, Melanoma, Uveal melanoma, Uvea, DecisionDx-UM, Draft:Carlos Rogerio Figueiredo
Annonce publiée le 14-01-2025
Institut Curie
Centre de recherche - Paris - Amphitheatre Constant-Burg - 12 rue Lhomond, Paris 5e
Mercredi 26 Mars 2025    0:00
Ce cours explorera la polyvalence des elements d ADN non geniques et des ARN non codants dans un large eventail de processus cellulaires, chez l humain et les organismes modeles, ainsi que leur implication dans la physiologie et les maladies. Il elargira les sujets autour des domaines de la genomique, de l epigenetique et de la transcriptomique, y compris les technologies et analyses a cellule unique, la regulation de l'epigenome et de l expression des genes, l organisation du genome et la clonalite cellulaire. Des experts reconnus a l international presenteront leurs dernieres decouvertes concernant l identification et la caracterisation fonctionnelle du genome non codant, et discuteront des nouveaux concepts en matiere de regulation et d evolution du genome, avec un fort accent sur les outils experimentaux et informatiques. Les sessions thematiques incluront l analyse computationnelle de l heterogeneite cellulaire, les methodes d evaluation de l heterogeneite et de la plasticite cellulaire, l epigenome dans la regulation de l expression des genes, les retroelements dans la plasticite cellulaire, le genome "sombre" dans l identite et la clonalite cellulaire, ainsi que l organisation spatiale. Ce cours offrira aux jeunes etudiants et chercheurs l opportunite d elargir leurs connaissances et de discuter de leurs travaux avec une communaute scientifique internationale dans un environnement chaleureux et stimulant a l Institut Curie a Paris. Keynote speakers Stein AERTS - BE Maria Elena TORRES PADILLA - DE Intervenants Tugce AKTAS - DE Maria BRBIC - CH Chunlong CHEN - FR Bart DEPLANCKE - CH Dominic GRÜN- DE Amit IDO - IL Jop KIND - NL Gioele LA MANNO - CH Ana POMBO - DE Alex RADZISHEUSKAYA - UK Alejo RODRIGUEZ FRATICELLI - ES Arjun RAJ - USA Arnau SEBRE-PEDROS - ES Sydney SHAFFER - USA Angela TADDEI - FR Barbara TREUTLEIN - CH Didier TRONO - CH Plus d'infos...
Annonce publiée le 06-12-2024
Institut Curie
Centre de recherche - Paris - Amphitheatre Helene Martel-Massignac (BDD)
Tags: Delayed open access journals, Cell, Cell and molecular biology, Transfersome
Annonce publiée le 21-12-2024
Institut Curie
Centre de recherche - Paris - Amphitheatre Helene Martel-Massignac (BDD)
Jeudi 10 Avril 2025    0:00
The development of complex in vitro models, such as organoids, gastruloids and organ-on-chips systems, will allow the better understanding of human biological processes that are otherwise difficult to address with classical in vitro 2D culture and/or with animal models. Elucidating how pathogens, such as the SARS-CoV-2, invade human cells by evading the immune system and how this could be modulated by the host microbiota has been greatly facilitated by the advancement of 3D cell culture techniques. For example, mimicking the gut peristalsis in gut-on-a-chip device improves the maturation of colon epithelial cells and aid to unveil the role of mechanical stress in accelerating enteropathogen invasion. Our lab is working on establishing unique advanced microphysiological systems that can mimic the interaction between human epithelial barriers with the surrounding tissues, such as blood vessels, mesenchyme and immune cells. My scientific project is focused on the establishment of lung-on-chip devices that cover the entire respiratory tract (from the nasopharynx to the alveoli) as a platform to understand airborne infections and tropism of respiratory viruses. There we relay both on the use of lung multipotent stem cells grown as organoids, in order to produce the different cell population of the respiratory tract, and on microfluidic chips. Paris Post-docs seminar series. Plus d'infos...