Le seminaire sera en francais More details... Published on 05-12-2023 Tweet |
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Institut Pasteur Batiment Fernbach, salle salle Jean-Paul Aubert, |
Le seminaire sera en francais More details... Published on 05-12-2023 Tweet |
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Institut Pasteur Batiment Fernbach, salle salle Jean-Paul Aubert, |
Life is highly dynamic relaying on a myriad of carefully orchestrated processes with strict spatial and temporal control. To decipher cellular functions and, in turn, address complex biological questions is necessary to have precise control. In my group, we pursue such the dream of achieving a molecularly precise understanding of biology to address the problems arising from misregulation. For this purpose, we focus on the design of innovative chemical biology tools. In particular, optochemical tools based on the synthesis of photosensitive molecules such as fluorophores, photosensitizers and photoswitches enabled us the desired conditional modulation in disease-related processes. In this talk I will make a brief summary about our projects where fluorophores have demonstrated the possibility of efficiently labelled multidrug resistant bacteria1 as well as the development of bioorthogonally activatable photosensitizers for photodynamic therapy.2-3 However, the main part of this presentation will deal with the use of photoswitches for light-controllable gene expression. All cells in an organism contain the same DNA sequence but vary greatly in gene expression. Epigenetics deals with these phenotype changes that retains the same DNA sequence. Importantly, misregulation of these epigenetic processes is implicated in the pathophysiology of numerous human diseases,4 including: cancer, autoimmune disorders and neurodegenerative disease. Therefore, epigenetic regulation is at the core of both natural and pathological states. The current available methods do not have sufficient spatiotemporal resolution to deal with the challenges of targeting the dynamic epigenome. We and others 5 envision that light could offer new possibilities and achieve molecular functionality. Reversible photoswitches, which have demonstrated their potential in diverse areas such as material science, have hardly implemented as genome regulators. Modulating the epigenome to tune transcription profiles, and cellular phenotypes in a programmable manner is of wide interest, and may ultimately lead to novel epigenetics-based therapeutics. Here, I will present our journey to achieve in vivo manipulation, 6-9 as well as our most recent work where photoswitches can control splicing and, in turn, gene expression.10
References 1. Schulte, L. N.; Heinrich, B.; Janga, H.; Schmeck, B. T.; Vazquez, O., A Far-Red Fluorescent DNA Binder for Interaction Studies of Live Multidrug-Resistant Pathogens and Host Cells. Angew Chem Int Ed Engl 2018, 57 (36), 11564-11568. 2. Linden, G.; Zhang, L.; Pieck, F.; Linne, U.; Kosenkov, D.; Tonner, R.; Vazquez, O., Conditional Singlet Oxygen Generation through a Bioorthogonal DNA-targeted Tetrazine Reaction. Angew Chem Int Ed Engl 2019, 58 (37), 12868-12873. 3. Linden, G.; Vazquez, O., Bioorthogonal Turn-On BODIPY-Peptide Photosensitizers for Tailored Photodynamic Therapy. Chemistry 2020, 26 (44), 10014-10023. 4. Portela, A.; Esteller, M., Epigenetic modifications and human disease. Nat Biotechnol 2010, 28 (10), 1057-68. 5. Hartmann, D.; Smith, J. M.; Mazzotti, G.; Chowdhry, R.; Booth, M. J., Controlling gene expression with light: a multidisciplinary endeavour. Biochem Soc Trans 2020, 48 (4), 1645-1659. 6. Albert, L.; Xu, J.; Wan, R.; Srinivasan, V.; Dou, Y.; Vazquez, O., Controlled inhibition of methyltransferases using photoswitchable peptidomimetics: towards an epigenetic regulation of leukemia. Chem Sci 2017, 8 (6), 4612-4618. 7. Heinrich, B.; Bouazoune, K.; Wojcik, M.; Bakowsky, U.; Vazquez, O., ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light. Org Biomol Chem 2019, 17 (7), 1827-1833. 8. Albert, L.; Penalver, A.; Djokovic, N.; Werel, L.; Hoffarth, M.; Ruzic, D.; Xu, J.; Essen, L. O.; Nikolic, K.; Dou, Y.; Vazquez, O., Modulating Protein-Protein Interactions with Visible-Light-Responsive Peptide Backbone Photoswitches. Chembiochem 2019, 20 (11), 1417-1429. 9. Albert, L.; Nagpal, J.; Steinchen, W.; Zhang, L.; Werel, L.; Djokovic, N.; Ruzic, D.; Hoffarth, M.; Xu, J.; Kaspareit, J.; Abendroth, F.; Royant, A.; Bange, G.; Nikolic, K.; Ryu, S.; Dou, Y.; Essen, L. O.; Vazquez, O., Bistable Photoswitch Allows in Vivo Control of Hematopoiesis. ACS Cent Sci 2022, 8 (1), 57-66. 10. Zhang, L.; Xie, X.; Djokovic, N.; Nikolic, K.; Kosenkov, D.; Abendroth, F.; Vazquez, O., Reversible Control of RNA Splicing by Photoswitchable Small Molecules. J Am Chem Soc 2023, 145 (23), 12783-12792. More details... Tags: Marburg Published on 04-12-2023 Tweet |
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Institut Curie Centre de recherche - Orsay - Amphitheatre du Batiment 111 |
TBA More details... Tags: Abbara, Films, ETH Domain, Alia, cole Polytechnique Fdrale de Lausanne Published on 02-12-2023 Tweet |
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Laboratoire Jean Perrin salle de seminaires 5eme etage - LJP - Tour 32-33 |
Le seminaire sera en francais More details... Published on 01-12-2023 Tweet |
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Institut Pasteur Batiment Fernbach, salle salle Jean-Paul Aubert, |
Invited by: IBENS - Equipe LENA-POPA More details... Tags: Implicit Published on 30-11-2023 Tweet |
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IBENS salle Favard, IBENS 46 rue d'Ulm 75005 Paris |
Invited by: Les Mercredi du CRI More details... Tags: Immunology, Innate immunity, Lung inflammation Published on 26-11-2023 Tweet |
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Centre de Recherche sur l'Inflammation salle 241B, Faculte de Medecine site Bichat, 16 rue Henri Huchard - 75018 |
Invited by: Reseau MicroLaser Biotech (RMLB) de l Association Francaise d Histotechnologie More details... Tags: Microdissection, Histology Published on 26-11-2023 Tweet |
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Institut Cochin salle Schapira, au 3eme etage de la Faculte de Medecine, 24 rue de Faubourg Saint Jacques - 75014 |
Invited by: Nienke Buddelmeijer Pr. Musa M. Mhlanga Radboud University, NetherlandsEpigenomics et Single Cell Biophysics GroupDept. of Cell Biology | Radboud Institute for Molecular Life SciencesDept. of Human Genetics | Radboud University Medical Center Epigenetic regulation: at the apex ... More details... Tags: Radboud University Medical Center, Radboud University Nijmegen, Epigenetics, Immunological memory, Radbod, Members of the Royal Netherlands Academy of Arts and Sciences Published on 25-11-2023 Tweet |
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Institut Pasteur Batiment Duclaux Building, salle Duclaux Amphitheater, |
Invited by: Aude Bernheim More details... Tags: Carrie Published on 25-11-2023 Tweet |
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Institut Pasteur Batiment: Centre F. Jacob Salle: Auditorium F. Jacob |
Invited by: Jean Christophe Poncer More details... Tags: Cajal, Prida, Journal of Neuroscience Methods, Science and technology in Spain, Cajal Institute Published on 23-11-2023 Tweet |
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Insitut du Fer à Moulin 17 Rue du Fer a Moulin - 75005 Paris |
Invited by: Stephane Nedelec More details... Tags: Dresden, Dresden University, Outline of Dresden Published on 23-11-2023 Tweet |
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Insitut du Fer à Moulin 17 Rue du Fer a Moulin - 75005 Paris |
Invited by: Pierre-Marie Lledo More details... Tags: Neurology, Michal Schwartz, Lymphatic system, Alzheimer's disease, Neuroscience Published on 23-11-2023 Tweet |
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Institut Pasteur Salle AMPHITHEATER DUCLAUX, |
Invited by: Ivo Gomperts Boneca More details... Published on 23-11-2023 Tweet |
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Institut Pasteur Batiment: Monod building Salle: Amphitheater Agnes Ullman |
Invited by: Michael White Short abstract : Insecticide resistance is now widespread across Africa but the exposure to sublethal insecticides and subsequent impact on the parasite development remains underexplored. In this talk I will discuss some ongoing work ... More details... Tags: Biocides, Disease ecology, Ecology, Parasitism, Parasitology, Insecticide, Insect Published on 23-11-2023 Tweet |
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Institut Pasteur Batiment Retrovirus, salle Lwoff, |
My scientific path started with the characterization of anabolic and catabolic circulating factors that associate with muscle wasting in several animal models of cancer cachexia, finding reduced IGF-1 and increased myostatin as common signs induced by tumor growth. My research provided the first evidence that tumor growth impairs the myogenic potential and that ERK inhibition rescues the myogenic capacity and partially prevents muscle depletion in tumor hosts. In parallel, I described the specific effects of tumor growth on muscle mitochondria and the ability of training exercise to revert such alterations in order to move the scientific interest from muscle mass to muscle quality, function, and metabolism. The current focus of my research has moved from a muscle centric perspective to a broad host metabolism approach, in order to understand cancer as a systemic host disease. More details... Tags: Rehabilitation medicine, Growth factors, Geriatrics, Aging-associated diseases, Muscular dystrophy, Myostatin, Cachexia, Skeletal muscle, Muscle atrophy, MTOR, Myosatellite cell, Insulin-like growth factor 1 Published on 22-11-2023 Tweet |
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Centre de Recherche en Myologie |
Invited by Suzanne Faure-Dupuy, Alberto De la Iglesia and Hugo Barreto, part of the Post-doc seminar series. Viral pathogens transmitted by mosquitoes represent a growing threat to human health, due to global warming and globalization. For example, Aedes aegypti (Ae. aegypti) mosquitoes are the main vectors of dengue viruses (DENV) that infect 400 million people annually. Previous studies showed that the ability of a given mosquito population to acquire and transmit a given virus is highly variable. The mechanisms underlying natural variation in susceptibility to viral infection between different mosquito populations remain mostly unknown. To explore this, we combined single-cell transcriptomics and metabolomics on Ae. aegypti populations showing contrasted infection phenotypes after DENV exposure. We identified Ae. aegypti mosquito populations, recently collected in the field, that are either resistant or susceptible to infection with DENV. When a mosquito bites a virus-infected human, the bloodmeal containing viral particles enters the mosquito’s digestive tract and is digested in the midgut, which is the entry gate for viruses into the mosquito’s body. Subsequently, the virus infects the fat body, the main immune and metabolic organ. We analyzed infection dynamics in those two mosquito organs and subjected them to single-cell RNA sequencing and tissue metabolomics analysis, in both resistant and susceptible Ae. aegypti populations. We described the metabolic functions of different cell subpopulations of the mosquito midgut and fat body upon DENV infection. We revealed metabolic pathways and gene expression patterns associated with susceptibility to virus infection at the organ and cellular levels. Results obtained from this study identify mosquito factors underlying natural variation to DENV infection and could lead to innovative tools for preventing arbovirus transmission. More details... Tags: Dengue fever, Tropical diseases, Flaviviruses, Zoonoses, Ectoparasites, Dengue virus, Aedes aegypti, Mosquito, Arbovirus, Disease vector, Stegomyia, Aedes Published on 21-11-2023 Tweet |
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Institut Cochin Salle Rosalind Franklin |
Invited by: Brice Bathellier Upcoming title Auditorium of the Hearing Institut 63 rue de Charenton 75012 Paris __ The organizing committee: Luc Arnal, Jeremie Barral, Brice Bathellier, Nicolas Michalski More details... Published on 18-11-2023 Tweet |
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Institut Pasteur Batiment I, salle , |
Androgens and glucocorticoids are steroid hormones that exert pleiotropic functions in mammals. Androgens has anabolic actions in tissues such as bone and muscle, and are crucial for the development and maintenance of reproductive organs in men. Since androgens stimulate prostatic epithelial cell proliferation and the growth of most prostate cancers, down regulation of androgen signaling is the major therapeutic strategy for advanced disease. Glucocorticoids control energy metabolism and the immune response. Synthetic glucocorticoids are widely used for their potent anti-inflammatory and immunosuppressive activities, and to manage symptoms and mitigate side effects induced by androgen deprivation therapy. However, long-term glucocorticoid treatments induce profound disturbances of intermediate metabolism, resulting in abdominal obesity, insulin resistance, and/or muscle wasting, and might confer prostate cancer anti-androgen resistance. Androgens and glucocorticoids mainly act via two members of the nuclear receptor superfamily, i.e. the androgen receptor (AR, Nr3c4) and the glucocorticoid receptor (GR, Nr3c1), respectively. To identify the molecular and cellular mechanisms by which these steroid hormones control muscle mass and function, we analyzed mice in which the cognate receptors are selectively ablated in myofibers, and performed extensive phenotypic and genome-wide analyses. Moreover, to provide insights into prostatic tumorigenesis and androgen signaling inhibition resistance, we generated genetically engineered mice that faithfully reproduce tumor progression in patients. The results gained from our recent studies will be presented. Daniel Metzger is invited by Pascal Maire. More details... Tags: Hepatotoxins, Sex hormones, Androgen, Antiandrogen, Glucocorticoid, Prostate, Steroid, Draft:Bipolar Androgen Therapy, Membrane androgen receptor Published on 16-11-2023 Tweet |
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Institut Cochin Salle Rosalind Franklin |
More details... Tags: Banking in India, Institute of Banking Personnel Selection, ESPCI Paris Published on 15-11-2023 Tweet |
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Inst. Bio. Paris Seine 7-9 quai Saint Bernard, 75005 Paris |
Invited by: CIRB - College de France More details... Tags: @CirbCdf Published on 15-11-2023 Tweet |
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College de France au College de France - Salle D2 11 place Marcelin Berthelot - 75005 Paris |
Kinetic X-ray crystallography permits the structural characterization of macromolecular conformational changes along a reaction pathway at the atomic level of spatial resolution. After triggering the biological reaction within a macromolecular crystal, functionally relevant conformational changes are either arrested by flash-cooling the crystal, allowing characterization of the structure by conventional cryo-crystallography (intermediate trapping), or followed in real time by time-resolved crystallography at room temperature. The temporal resolution of the latter is limited to 100 ps if carried out in the form of Laue crystallography at synchrotrons. The advent of X-ray free electron lasers (XFELs) has pushed the resolution to the sub-ps regime, allowing ultrafast changes to be studied by time-resolved serial femtosecond crystallography. After reviewing the current status of the field, we will illustrate the time-resolved crystallography approach with the study of photoisomerization of the chromophore in a reversibly photoswitchable fluorescent protein. We will indicate how information on ultrafast structural dynamics can be used to rationally modify photoswitchable fluorescent proteins for applications in biological imaging. Published on 14-11-2023 Tweet |
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Ecole Polytechnique Amphi Curie |
Invited by: Jean Christophe Poncer More details... Tags: Genomics, Omics, Biotechnology, Microscopy Published on 09-11-2023 Tweet |
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Insitut du Fer à Moulin 17 Rue du Fer a Moulin - 75005 Paris |
TBA More details... Tags: Wolfram, Holy Grail, Middle High German literature, Conrad Wolfram, Catherine Wolfram Published on 08-11-2023 Tweet |
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Laboratoire Jean Perrin salle de seminaires 5eme etage - LJP - Tours 32-33 |
Invited by: Julien Ferent More details... Published on 08-11-2023 Tweet |
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Insitut du Fer à Moulin 17 Rue du Fer a Moulin - 75005 Paris |
Invited by: Gabrielle Girardeau More details... Tags: Wellcome Trust, Russell Group, Universities UK, University College London, University of London, UCL, UCL Faculty of Mathematical and Physical Sciences, UCL Faculty of Life Sciences Published on 08-11-2023 Tweet |
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Insitut du Fer à Moulin 17 Rue du Fer a Moulin - 75005 Paris |
Invited by: Nienke Buddelmeijer Pr. Musa M. Mhlanga Epigenetic regulation: at the apex of Innate Immune Memory December 14th, 2023 - 11am Duclaux Amphitheatre - Duclaux Building INTRODUCTION Musa M. Mhlanga Ph.D chairs the department of Cell Biology ... More details... Tags: Immune system, Immunological memory, Epigenetics Published on 28-10-2023 Tweet |
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Institut Pasteur Batiment Duclaux Building, salle Duclaux Amphitheater, |
Invited by: Jean Christophe Poncer More details... Tags: Cajal, Prida, Journal of Neuroscience Methods, Science and technology in Spain, Cajal Institute Published on 18-10-2023 Tweet |
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Insitut du Fer à Moulin 17 Rue du Fer a Moulin - 75005 Paris |
Invited by: VASILIAUSKAS Daniel More details... Tags: McGill University, U15 Group of Canadian Research Universities, McGill, Officers of the Order of Canada, Theodore Sourkes Published on 10-10-2023 Tweet |
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NeuroPSI Salle de conference Albe-Fessard |
Invited by: CIRB - College de France More details... Tags: @CirbCdf Published on 09-10-2023 Tweet |
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College de France au College de France - Salle D2 11 place Marcelin Berthelot - 75005 Paris |
Invited by: CIRB - College de France More details... Tags: @CirbCdf Published on 09-10-2023 Tweet |
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College de France au College de France - Salle D2 11 place Marcelin Berthelot - 75005 Paris |
Invited by: CIRB - College de France More details... Tags: @CirbCdf Published on 09-10-2023 Tweet |
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College de France au College de France - Salle D2 11 place Marcelin Berthelot - 75005 Paris |
Invited by: CIRB - College de France More details... Tags: @CirbCdf Published on 26-07-2023 Tweet |
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College de France au College de France - Salle D2 11 place Marcelin Berthelot - 75005 Paris |
Invited by: CREUZET Sophie More details... Tags: Martine Published on 25-07-2023 Tweet |
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NeuroPSI Salle de conference Albe-Fessard |
TBA More details... Tags: Grandes coles, Ens, cole normale suprieure, Draft:202526 Indian Super League, cole normale suprieure de Rennes Published on 02-07-2023 Tweet |
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Laboratoire Jean Perrin Salle de seminaires 5eme etage - LJP - Tours 32-33 |
L'objectif de ce cours est de fournir une vue d'ensemble pratique et actualisée des dernières recherches et des développements cliniques dans le domaine du cancer urothélial, y compris les cancers de la vessie invasifs et non invasifs sur le plan musculaire, les carcinomes urothéliaux des voies urinaires supérieures et leurs métastases. Ce faisant, nous visons à : a) promouvoir une compréhension consensuelle des stratégies optimales actuelles de traitement de ces maladies, b) fournir des connaissances pratiques et une perspective clinique des maladies et de leur traitement, c) promouvoir une meilleure compréhension mécaniste des maladies et des méthodologies utilisées pour les étudier, et d) fournir une perspective industrielle pour partager la façon dont les nouveaux traitements sont développés. More details... Published on 12-06-2023 Tweet |
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Institut Curie Centre de recherche - Paris - Amphitheatre Helene Martel-Massignac (BDD) |
Invited by Pascal Maire. More details... Tags: Illkirch-Graffenstaden, Maire Published on 10-06-2023 Tweet |
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Institut Cochin Salle Rosalind Franklin |
More details... Tags: cne, Revue de Mdecine Interne Published on 15-05-2023 Tweet |
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Laboratoire Jean Perrin Salle de seminaires 5eme etage - LJP - Tours 32-33 |
Cell plasticity plays an essential role in chronic pancreatitis, human pancreatic cancer (PDAC) and development of diabetes. The morphological processes are closely related to molecular programs known from early embryonic development of the pancreas. Based on these analogies, we have developed new disease models to decipher the causes of the aforementioned pathophysiologies and to design innovative, personalized therapies. One of the core areas in the lab is pancreatic differentiation of human pluripotent stem cells (hPSC). Here , we have differentiated hPSCs into pancreatic duct-like organoids (PDLOs) with morphological, transcriptional, proteomic, and functional characteristics of human pancreatic ducts, further maturing upon transplantation into mice. This model enabled in vitro and in vivo studies of pancreatic plasticity, dysplasia, and cancer formation from a genetically defined background. Using similar models, we also approached the endocrine lineage and revealed that loss of ONECUT1 impairs pancreatic progenitor formation and a subsequent endocrine program. Loss of ONECUT1 altered transcription factor binding and enhancer activity and NKX2.2/NKX6.1 expression in pancreatic progenitor cells. Collectively, we demonstrate that ONECUT1 controls a transcriptional and epigenetic machinery regulating endocrine development, involved in a spectrum of diabetes, encompassing monogenic (recessive and dominant) as well as multifactorial inheritance. In this seminar, I will summarize these seminal papers and give an overview about our unpublished efforts to model pancreatic disorders. Invited by Cécile Julier More details... Tags: Branches of biology, Biology, Life sciences, Stem cells, Developmental biology, Biotechnology, Induced stem cells, Cell biology, Organoid, Pancreatic progenitor cell, Pancreas, Progenitor cell Published on 15-05-2023 Tweet |
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Institut Cochin Salle Rosalind Franklin |
More details... Tags: Digital PCR, Virology, Infectious disease Published on 13-01-2023 Tweet |
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Centre de Recherche des Cordeliers amphitheatre Roussy, 15 rue de l'Ecole de Medecine 75006 |
More details... Tags: Draft:Rajat Gupta kanpur, Ada Twist, Scientist Published on 07-11-2021 Tweet |
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NeuroPSI Salle de conference Albe-Fessard |